Abstract: The shortcomings of standard plasma-separation methods limit the point-of-care application\nof microfluidics in clinical facilities and at the patientâ??s bedside. To overcome the limitations of this\ninconvenient, laborious, and costly technique, a new plasma-separation technique and device were\ndeveloped. This new separation method relies on immunological capture and filtration to exclude cells\nfrom plasma, and is convenient, easy to use, and cost-effective. Most of the RBCs can be captured and\nimmobilized by antibody which coated in separation matrix, and residue cells can be totally removed\nfrom the sample by a commercially plasma purification membranes. A 400 MicroL anti-coagulated whole\nblood sample with 65% hematocrit (Hct) can be separated by the device in 5 min with only one pipette.\nUp to 97% of the plasma can be recovered from the raw blood sample with a separation efficiency\nat 100%. The recovery rate of small molecule compounds, proteins, and nucleic acid biomarkers is\nevaluated; there are no obvious differences from the centrifuge method. The results demonstrate that\nthis method is an excellent replacement for traditional plasma preparation protocols.
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